Z3EV
| Type: | Synthetic promoter |
| Other names: | Estradiol-inducible system; hormone-inducible system |
| Description: | Z3EV is a transcription factor in which the estradiol receptor is fused to the DNA binding domain of the mouse transcription factor Zif268 and the transcriptional activation domain VP161. One of the main advantages of using artificial transcription factors is that they can be designed to recognize comparatively long DNA motifs, thereby reducing off-target binding. |
| Organism of origin: | The activation domain of the synthetic transcription factor comes from human (Homo sapiens sapiens) estrogen receptor, the DNA binding domain of the transcription factor comes from mouse (Mus musculus) while the fused VP16 activation domain comes from herpes virus (Herpes simplex) |
| Available on Addgene: | https://www.addgene.org/69099/ |
| Inducing condition: | Presence of estradiol |
| Repressing condition: | Absence of estradiol |
Promoter name Leakiness [% maxGAL1] tau-on [min] tau-off [min] Maximal induction level [maxGAL1] Stationary induction level [maxGAL1] Initial induction speed [0.001 maxGAL1/min] Degradation rate [0.01/min]
Z3EV 0.16 7.1 0.52 0.44 2.6 0.1
References:
- McIsaac, R. S., Gibney, P. A., Chandran, S. S., Benjamin, K. R. & Botstein, D. Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae. Nucleic Acids Res. 42, e48–e48 (2014). https://doi.org/10.1093/nar/gkt1402