GLIP-EL222
| Type: | Synthetic promoter; Optogenetic promoter |
| Other names: | / |
| Description: | GLIP (“GAL1pr-based light-inducible promoter”) is another light-inducible promoter, induced by El222 transcription factor1. GLIP was constructed from GAL1pr by replacing the Gal4 activator binding sites with El222 binding sites2 |
| Organism of origin: | The light-responsive prokaryotic transcription factor EL222 and its binding sites come from Erythrobacter litoralis. The transactivation domain which adapts it for eukaryotes comes from the herpes virus (Herpes simplex) protein VP16. |
| Available on Addgene: | / |
| Inducing condition: | Blue light (≈ 465 nm) |
| Non-inducing condition: | Darkness |
Promoter name Leakiness [% maxGAL1] tau-on [min] tau-off [min] Maximal induction level [maxGAL1] Stationary induction level [maxGAL1] Initial induction speed [0.001 maxGAL1/min] Degradation rate [0.01/min]
GLIP-EL222 0.01 2.7 7.6 0.13 0.10 1.6 1.3
References:
- Motta-Mena, L. B., Reade, A., Mallory, M. J., Glantz, S., Weiner, O. D., Lynch, K. W. & Gardner, K. H. An optogenetic gene expression system with rapid activation and deactivation kinetics. Nat. Chem. Biol. 10, 196–202 (2014). https://doi.org/10.1038/nchembio.1430
- Benzinger, D. & Khammash, M. Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation. Nat. Commun. 9, 3521 (2018). https://doi.org/10.1038/s41467-018-05882-2