LIP-strongLOV
| Type: | Synthetic promoter; Optogenetic promoter |
| Other names: | / |
| Description: | strongLOV is the more light-sensitive Glu84Asp mutant of the El222 transcription factor.1. |
| Organism of origin: | The light-responsive prokaryotic transcription factor EL222 and its binding sites come from Erythrobacter litoralis. The transactivation domain which adapts it for eukaryotes comes from the herpes virus (Herpes simplex) protein VP16. |
| Available on Addgene: | / |
| Inducing condition: | Blue light (≈ 465 nm) |
| Non-inducing condition: | Darkness |
Promoter name Leakiness [% maxGAL1] tau-on [min] tau-off* [min] Maximal induction level [maxGAL1] Stationary induction level [maxGAL1] Initial induction speed [0.001 maxGAL1/min] Degradation rate*[0.01/min]
LIP-EL222 80% 0.16 2.2 12.6 0.73 0.60 3.1 1.1
LIP-strongLOV 20% 0.80 1.4 46.2 0.58 0.56 2.6 1.6
LIP-EL222 20% 0.16 1.3 < 1 0.20 0.16 1.3 1.0
*t-off and degradation rate were determined using ymScarletI as a reporter, instead of yEVenus as for the other systems. This was done because light for yEVenus excitation was strong enough to induce strongLOV.
References:
- Gligorovski, V., Sadeghi, A., Rahi, S. J. “Multidimensional single-cell benchmarking of inducible promoters for precise dynamic control in budding yeast”, bioRxiv preprint 2020. https://doi.org/10.1101/2020.08.16.253310