PHO5pr
| Type: | Metabolite-inducible promoter |
| Other names: | / |
| Description: | PHO5pr is a member of the PHO regulon, which has been researched intensively as a model for studying the relationship between chromatin structure and gene expression dynamics1. PHO5pr is upregulated in response to a lack of inorganic phosphate2, which is required for energy and nucleotide metabolism. The PHO5 promoter becomes fully active after phosphate, including any stored in the vacuole, is used up3. |
| Organism of origin: | budding yeast (Saccharomyces cerevisiae) |
| Available on Addgene: | https://www.addgene.org/164704/ |
| Inducing condition: | Lack of inorganic phosphate |
| Repressible condition: | Inorganic phosphate |
Promoter name Leakiness [% maxGAL1] tau-on [min] tau-off [min] Maximal induction level [maxGAL1] Stationary induction level [maxGAL1] Initial induction speed [0.001 maxGAL1/min] Degradation rate [0.01/min]
PHO5pr 0.06 20.0 0.33 0.31 1.1 1.2
References:
- Korber, P. & Barbaric, S. The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin. Nucleic Acids Res. 42, 10888–10902 (2014). https://doi.org/10.1093/nar/gku784
- Lenburg, M. & Oshea, E. Signaling phosphate starvation. Trends Biochem. Sci. 21, 383–387 (1996). https://doi.org/10.1016/S0968-0004(96)10048-7
- Thomas, M. R. & O’Shea, E. K. An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels. Proc. Natl. Acad. Sci. 102, 9565–9570 (2005). https://doi.org/10.1073/pnas.0501122102