MET3pr
| Type: | Metabolite-inducible promoter |
| Other names: | / |
| Description: | MET3 was discovered through a screen for methionine auxotrophy in yeast1. Met3 is an ATP-sulfurylase which catalyzes the first step in the sulfur assimilation pathway.2 Its transcription is strongly repressed in methionine-rich media3. It is commonly used to control the expression of budding yeast genes whose transcription levels are lower than GAL1pr. |
| Organism of origin: | budding yeast (Saccharomyces cerevisiae) |
| Available on Addgene: | https://www.addgene.org/164710/ |
| Inducing condition: | Lack of methionine |
| Non-inducing condition: | Methionine |
Promoter name Leakiness [% maxGAL1] tau-on [min] tau-off [min] Maximal induction level [maxGAL1] Stationary induction level [maxGAL1] Initial induction speed [0.001 maxGAL1/min] Degradation rate [0.01/min]
MET3pr 0.08 13.9 < 1 0.18 0.11 3.0 1.7
References:
- Cherest, H., Eichler, F. & de Robichon-Szulmajster, H. Genetic and regulatory aspects of methionine biosynthesis in Saccharomyces cerevisiae. J. Bacteriol. 97, 328–336 (1969). https://doi.org/10.1128/jb.97.1.328-336.1969
- Gierest, H., Thao, N. N. & Surdin-Kerjan, Y. Transcriptional regulation of the MET3 gene of Saccharomyces cerevisiae. Gene 34, 269–281 (1985). https://doi.org/10.1016/0378-1119(85)90136-2
- Charvin, G., Cross, F. R. & Siggia, E. D. A microfluidic device for temporally controlled gene expression and long-term fluorescent imaging in unperturbed dividing yeast cells. PLoS ONE 3, e1468 (2008). https://doi.org/10.1371%2Fjournal.pone.0001468