CUP1pr
| Type: | Metabolite-inducible promoter |
| Other names: | / |
| Description: | CUP1 is part of a feedback loop that mediates resistance to copper toxicity. Its transcription increases when cells are exposed to copper (II) ions.1 Although CUP1pr has been used as a tool for dynamic gene expression control, it has the fundamental drawback of being regulated by copper ions, which are both essential and, if supplied at high concentrations, toxic.2 |
| Organism of origin: | Budding yeast (Saccharomyces cerevisiae) |
| Available on Addgene: | https://www.addgene.org/164703/ |
| Inducing condition: | Copper(II) ions |
| Non-inducing condition: | Lack of copper(II) ions |
Promoter name Leakiness [% maxGAL1] tau-on [min] tau-off [min] Maximal induction level [maxGAL1] Stationary induction level [maxGAL1] Initial induction speed [0.001 maxGAL1/min] Degradation rate [0.01/min]
CUP1pr 1.09 3.6 2.7 0.10 0.07 4.1 0.6
References:
- Fürst, P., Hu, S., Hackett, R. & Hamer, D. Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein. Cell 55, 705–717 (1988). 10.1016/0092-8674(88)90229-2
- Mascorro-Gallardo, J. O., Covarrubias, A. A. & Gaxiola, R. Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae. Gene 172, 169–170 (1996). 10.1016/0378-1119(96)00059-5