Tet-On system
| Type: | Synthetic promoter |
| Other names: | Tetracycline-inducible system; doxycycline-inducible system |
| Description: | Tetracycline-responsive systems are widely used for controlling transcription. The core of the system, the tetO sequence, is controlled by the tTA regulator, which has been identified as a tetracycline-responsive element in bacteria. In its original form, tTA is part of the Tet-Off system that is inhibited by the antibiotic tetracycline or the closely related molecule doxycycline1. The Tet-On system was created through mutations that reverse the activity of tTA in the presence and absence of tetracycline/doxycycline2. |
| Organism of origin: | The tetracycline-regulated parts of the system (tetO and tTA) come from the Tn10 transposon of Escherichia coli |
| Available on Addgene: | https://www.addgene.org/164705/ |
| Inducing condition: | Presence of tetracycline |
| Repressing condition: | Absence of tetracycline |
Promoter name Leakiness* [% maxGAL1] tau-on [min] tau-off* [min] Maximal induction level [maxGAL1] Stationary induction level [maxGAL1] Initial induction speed [0.001 maxGAL1/min] Degradation rate [0.01/min]
tetO 1.00 8.5 31.6 0.31 0.19 1.3 0.6
References:
- Garí, E., Piedrafita, L., Aldea, M. & Herrero, E. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast Chichester Engl. 13, 837–848 (1997). https://doi.org/10.1002/(sici)1097-0061(199707)13:9%3C837::aid-yea145%3E3.0.co;2-t
- Belli, G., Garí, E., Piedrafita, L., Aldea, M. & Herrero, E. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. Nucleic Acids Res. 26, 942–947 (1998). https://doi.org/10.1093%2Fnar%2F26.4.942