{"id":919,"date":"2022-08-06T16:41:01","date_gmt":"2022-08-06T16:41:01","guid":{"rendered":"https:\/\/promoter-benchmark.epfl.ch\/?page_id=919"},"modified":"2023-06-27T19:54:02","modified_gmt":"2023-06-27T19:54:02","slug":"lip-el222-system","status":"publish","type":"page","link":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/lip-el222-system\/","title":{"rendered":"LIP-EL222"},"content":{"rendered":"

LIP-EL222<\/span><\/strong><\/span><\/h1>\n
\n\n\n\n\n\n\n\n\n\n
Type:<\/span><\/td>\nSynthetic promoter; Optogenetic promoter<\/span><\/td>\n<\/tr>\n
Other names:<\/span><\/td>\n\/<\/span><\/td>\n<\/tr>\n
Description:<\/span><\/td>\nEl222 is a prokaryotic transcription factor that contains a light responsive LOV domain. It has been adapted for use in many organisms such as yeast, zebrafish, and mammalian cell lines1,2,3<\/sup>\u00a0by fusing it to the transcriptional activation domain VP16 and a nuclear localization sequence. When exposed to blue light (\u2248 465 nm), El222 dimerizes and recognizes its binding sites4<\/sup>. These binding sites are typically placed upstream of a minimal promoter1,2<\/sup>. We refer to the whole promoter, introduced in ref.2<\/sup> as LIP<\/em> (\u201cl<\/u>ight-i<\/u>nducible p<\/u>romoter\u201d).\u00a0 El222 incorporates flavin-mononucleotide as chromophore, which is naturally occurring in budding yeast. A recent version of LIP<\/em> has been built using the GAL1<\/em> promoter with the Gal4 activator binding sites deleted5<\/sup> instead of the minimal promoter, which we refer to as GLIP<\/em><\/a> (\u201cG<\/u>AL1pr-based l<\/u>ight-i<\/u>nducible p<\/u>romoter\u201d).<\/span><\/td>\n<\/tr>\n
Organism of origin:<\/span><\/td>\nThe light-responsive prokaryotic transcription factor EL222 and its binding sites come from Erythrobacter litoralis<\/em>. The transactivation domain which adapts it for eukaryotes comes from the herpes virus (Herpes simplex<\/em>) protein VP16.<\/span><\/td>\n<\/tr>\n
Available on Addgene:<\/span><\/td>\n\/<\/span><\/td>\n<\/tr>\n
Inducing condition:<\/span><\/td>\nBlue light (\u2248 465 nm)<\/span><\/td>\n<\/tr>\n
Non-inducing condition:<\/span><\/td>\nDarkness<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n\t\n\n\t\n\t
Promoter name<\/th>Leakiness [% maxGAL1]<\/th>tau-on [min]<\/th>tau-off* [min]<\/th>Maximal induction level [maxGAL1]<\/th>Stationary induction level [maxGAL1]<\/th>Initial induction speed [0.001 maxGAL1\/min]<\/th>Degradation rate* [0.01\/min]<\/th>\n<\/tr>\n<\/thead>\n
LIP-EL222 80%<\/td>0.16<\/td>2.2<\/td>12.6<\/td>0.73<\/td>0.60<\/td>3.1<\/td>1.1<\/td>\n<\/tr>\n
LIP-EL222 20%<\/td>0.16<\/td>1.3<\/td>< 1<\/td>0.20<\/td>0.16<\/td>1.3<\/td>1.0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/span><\/p>\n

*t-off and degradation rate were determined using ymScarletI as a reporter.<\/p>\n

Download promoter sequence<\/a><\/strong><\/span><\/p>\n

References:<\/span><\/p>\n

    \n
  1. Zhao, E. M., Zhang, Y., Mehl, J., Park, H., Lalwani, M. A., Toettcher, J. E. & Avalos, J. L. Optogenetic regulation of engineered cellular metabolism for microbial chemical production. Nature<\/em> 555,<\/strong> 683\u2013687 (2018). https:\/\/doi.org\/10.1038\/nature26141<\/a><\/span><\/li>\n
  2. Motta-Mena, L. B., Reade, A., Mallory, M. J., Glantz, S., Weiner, O. D., Lynch, K. W. & Gardner, K. H. An optogenetic gene expression system with rapid activation and deactivation kinetics. Nat. Chem. Biol.<\/em> 10,<\/strong> 196\u2013202 (2014). https:\/\/doi.org\/10.1038\/nchembio.1430<\/a><\/span><\/li>\n
  3. Reade, A., Motta-Mena, L. B., Gardner, K. H., Stainier, D. Y., Weiner, O. D. & Woo, S. TAEL: A zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control. Development<\/em> dev.139238 (2016). doi:10.1242\/dev.139238 https:\/\/doi.org\/10.1242\/dev.139238<\/a><\/span><\/li>\n
  4. Zoltowski, B. D., Motta-Mena, L. B. & Gardner, K. H. Blue light-induced dimerization of a bacterial LOV\u2013HTH DNA-binding protein. Biochemistry<\/em> 52,<\/strong> 6653\u20136661 (2013). https:\/\/doi.org\/10.1021\/bi401040m<\/a><\/span><\/li>\n
  5. Benzinger, D. & Khammash, M. Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation. Nat. Commun.<\/em> 9,<\/strong> 3521 (2018). https:\/\/doi.org\/10.1038\/s41467-018-05882-2<\/a><\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    LIP-EL222 Type: Synthetic promoter; Optogenetic promoter Other names: \/ Description: El222 is a prokaryotic transcription factor that contains a light responsive LOV domain. It has been adapted for use in many organisms such as yeast, zebrafish, and mammalian cell lines1,2,3\u00a0by fusing it to the transcriptional activation domain VP16 and a nuclear localization sequence. When exposed […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","footnotes":""},"class_list":["post-919","page","type-page","status-publish","hentry","entry"],"featured_image_src":null,"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages\/919","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/comments?post=919"}],"version-history":[{"count":0,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages\/919\/revisions"}],"wp:attachment":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/media?parent=919"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}