{"id":425,"date":"2022-08-02T12:12:48","date_gmt":"2022-08-02T12:12:48","guid":{"rendered":"https:\/\/promoter-benchmark.epfl.ch\/?page_id=425"},"modified":"2023-06-27T20:00:33","modified_gmt":"2023-06-27T20:00:33","slug":"tet-on-system","status":"publish","type":"page","link":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/tet-on-system\/","title":{"rendered":"tet-on"},"content":{"rendered":"

Tet-On system<\/span><\/strong><\/span><\/h1>\n
\n\n\n\n\n\n\n\n\n\n
Type:<\/span><\/td>\nSynthetic promoter<\/span><\/td>\n<\/tr>\n
Other names:<\/span><\/td>\nTetracycline-inducible system; doxycycline-inducible system<\/span><\/td>\n<\/tr>\n
Description:<\/span><\/td>\nTetracycline-responsive systems are widely used for controlling transcription. The core of the system, the tetO<\/em> sequence, is controlled by the tTA regulator, which has been identified as a tetracycline-responsive element in bacteria. In its original form, tTA is part of the Tet-Off system that is inhibited by the antibiotic tetracycline or the closely related molecule doxycycline1<\/sup>. The Tet-On system was created through mutations that reverse the activity of tTA in the presence and absence of tetracycline\/doxycycline2<\/sup>.<\/span><\/td>\n<\/tr>\n
Organism of origin:<\/span><\/td>\nThe tetracycline-regulated parts of the system (tetO and tTA) come from the Tn10 transposon of Escherichia coli<\/em><\/span><\/td>\n<\/tr>\n
Available on Addgene:<\/span><\/td>\nhttps:\/\/www.addgene.org\/164705\/<\/a><\/span><\/td>\n<\/tr>\n
Inducing condition:<\/span><\/td>\nPresence of tetracycline<\/span><\/td>\n<\/tr>\n
Repressing condition:<\/span><\/td>\nAbsence of tetracycline<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n\t\n\n\t
Promoter name<\/th>Leakiness* [% maxGAL1]<\/th>tau-on [min]<\/th>tau-off* [min]<\/th>Maximal induction level [maxGAL1]<\/th>Stationary induction level [maxGAL1]<\/th>Initial induction speed [0.001 maxGAL1\/min]<\/th>Degradation rate [0.01\/min]<\/th>\n<\/tr>\n<\/thead>\n
tetO<\/td>1.00<\/td>8.5<\/td>31.6<\/td>0.31<\/td>0.19<\/td>1.3<\/td>0.6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/span><\/p>\n

Download promoter sequence<\/a><\/span><\/strong><\/span><\/p>\n

References:<\/span><\/span><\/p>\n

    \n
  1. Gar\u00ed, E., Piedrafita, L., Aldea, M. & Herrero, E. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae<\/em>. Yeast Chichester Engl.<\/em> 13,<\/strong> 837\u2013848 (1997). https:\/\/doi.org\/10.1002\/(sici)1097-0061(199707)13:9%3C837::aid-yea145%3E3.0.co;2-t<\/a><\/span><\/li>\n
  2. Belli, G., Gar\u00ed, E., Piedrafita, L., Aldea, M. & Herrero, E. An activator\/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. Nucleic Acids Res.<\/em> 26,<\/strong> 942\u2013947 (1998). https:\/\/doi.org\/10.1093%2Fnar%2F26.4.942<\/a>
    \n<\/span><\/span><\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Tet-On system Type: Synthetic promoter Other names: Tetracycline-inducible system; doxycycline-inducible system Description: Tetracycline-responsive systems are widely used for controlling transcription. The core of the system, the tetO sequence, is controlled by the tTA regulator, which has been identified as a tetracycline-responsive element in bacteria. In its original form, tTA is part of the Tet-Off system […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","footnotes":""},"class_list":["post-425","page","type-page","status-publish","hentry","entry"],"featured_image_src":null,"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages\/425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/comments?post=425"}],"version-history":[{"count":0,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages\/425\/revisions"}],"wp:attachment":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/media?parent=425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}