{"id":423,"date":"2022-08-02T12:12:27","date_gmt":"2022-08-02T12:12:27","guid":{"rendered":"https:\/\/promoter-benchmark.epfl.ch\/?page_id=423"},"modified":"2023-06-27T19:59:12","modified_gmt":"2023-06-27T19:59:12","slug":"cup1-promoter","status":"publish","type":"page","link":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/cup1-promoter\/","title":{"rendered":"CUP1pr"},"content":{"rendered":"

CUP1pr<\/span><\/strong><\/span><\/h1>\n
\n\n\n\n\n\n\n\n\n\n
Type:<\/span><\/td>\nMetabolite-inducible promoter<\/span><\/td>\n<\/tr>\n
Other names:<\/span><\/td>\n\/<\/span><\/td>\n<\/tr>\n
Description:<\/span><\/td>\nCUP1 is part of a feedback loop that mediates resistance to copper toxicity. Its transcription increases when cells are exposed to copper (II) ions.1<\/sup> Although CUP1pr<\/em> has been used as a tool for dynamic gene expression control, it has the fundamental drawback of being regulated by copper ions, which are both essential and, if supplied at high concentrations, toxic.2<\/sup>\u00a0<\/span><\/td>\n<\/tr>\n
Organism of origin:<\/span><\/td>\nBudding yeast (Saccharomyces cerevisiae<\/em>)<\/span><\/td>\n<\/tr>\n
Available on Addgene:<\/span><\/td>\nhttps:\/\/www.addgene.org\/164703\/<\/a><\/span><\/td>\n<\/tr>\n
Inducing condition:<\/span><\/td>\nCopper(II) ions<\/span><\/td>\n<\/tr>\n
Non-inducing condition:<\/span><\/td>\nLack of copper(II) ions<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n\t\n\n\t
Promoter name<\/th>Leakiness [% maxGAL1]<\/th>tau-on [min]<\/th>tau-off [min]<\/th>Maximal induction level [maxGAL1]<\/th>Stationary induction level [maxGAL1]<\/th>Initial induction speed [0.001 maxGAL1\/min]<\/th>Degradation rate [0.01\/min]<\/th>\n<\/tr>\n<\/thead>\n
CUP1pr<\/td>1.09<\/td>3.6<\/td>2.7<\/td>0.10<\/td>0.07<\/td>4.1<\/td>0.6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/span><\/p>\n

Download promoter sequence<\/a><\/span><\/strong><\/span>
\n<\/span><\/strong><\/span><\/p>\n

References:<\/span><\/span><\/p>\n

    \n
  1. F\u00fcrst, P., Hu, S., Hackett, R. & Hamer, D. Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein. Cell<\/em> 55, 705\u2013717 (1988).\u00a010.1016\/0092-8674(88)90229-2<\/a><\/span><\/span><\/li>\n
  2. Mascorro-Gallardo, J. O., Covarrubias, A. A. & Gaxiola, R. Construction of a CUP1<\/em> promoter-based vector to modulate gene expression in Saccharomyces cerevisiae<\/em>. Gene<\/em> 172, 169\u2013170 (1996).\u00a010.1016\/0378-1119(96)00059-5<\/a><\/span><\/span><\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    CUP1pr Type: Metabolite-inducible promoter Other names: \/ Description: CUP1 is part of a feedback loop that mediates resistance to copper toxicity. Its transcription increases when cells are exposed to copper (II) ions.1 Although CUP1pr has been used as a tool for dynamic gene expression control, it has the fundamental drawback of being regulated by copper […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","footnotes":""},"class_list":["post-423","page","type-page","status-publish","hentry","entry"],"featured_image_src":null,"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t