{"id":421,"date":"2022-08-02T12:12:07","date_gmt":"2022-08-02T12:12:07","guid":{"rendered":"https:\/\/promoter-benchmark.epfl.ch\/?page_id=421"},"modified":"2023-06-27T19:56:35","modified_gmt":"2023-06-27T19:56:35","slug":"pho5-promoter","status":"publish","type":"page","link":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/pho5-promoter\/","title":{"rendered":"PHO5pr"},"content":{"rendered":"

PHO5pr<\/span><\/strong><\/span><\/h1>\n
\n\n\n\n\n\n\n\n\n\n
Type:<\/span><\/td>\nMetabolite-inducible promoter<\/span><\/td>\n<\/tr>\n
Other names:<\/span><\/td>\n\/<\/span><\/td>\n<\/tr>\n
Description:<\/span><\/td>\nPHO5pr<\/em> is a member of the PHO<\/em> regulon, which has been researched intensively as a model for studying the relationship between chromatin structure and gene expression dynamics1<\/sup>. PHO5pr<\/em> is upregulated in response to a lack of inorganic phosphate2<\/sup>, which is required for energy and nucleotide metabolism. The PHO5<\/em> promoter becomes fully active after phosphate, including any stored in the vacuole, is used up3<\/sup>.<\/span><\/td>\n<\/tr>\n
Organism of origin:<\/span><\/td>\nbudding yeast (Saccharomyces cerevisiae<\/em>)<\/span><\/td>\n<\/tr>\n
Available on Addgene:<\/span><\/td>\nhttps:\/\/www.addgene.org\/164704\/<\/a><\/span><\/td>\n<\/tr>\n
Inducing condition:<\/span><\/td>\nLack of inorganic phosphate<\/span><\/td>\n<\/tr>\n
Repressible condition:<\/span><\/td>\nInorganic phosphate<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n\t\n\n\t
Promoter name<\/th>Leakiness [% maxGAL1]<\/th>tau-on [min]<\/th>tau-off [min]<\/th>Maximal induction level [maxGAL1]<\/th>Stationary induction level [maxGAL1]<\/th>Initial induction speed [0.001 maxGAL1\/min]<\/th>Degradation rate [0.01\/min]<\/th>\n<\/tr>\n<\/thead>\n
PHO5pr<\/td>0.06<\/td>20.0<\/td><\/td>0.33<\/td>0.31<\/td>1.1<\/td>1.2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/span><\/p>\n

Download promoter sequence<\/a><\/span><\/strong><\/span><\/p>\n

References:<\/span><\/span><\/p>\n

    \n
  1. Korber, P. & Barbaric, S. The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin. Nucleic Acids Res.<\/em> 42,<\/strong> 10888\u201310902 (2014). https:\/\/doi.org\/10.1093\/nar\/gku784<\/a><\/span><\/li>\n
  2. Lenburg, M. & Oshea, E. Signaling phosphate starvation. Trends Biochem. Sci.<\/em> 21,<\/strong> 383\u2013387 (1996). https:\/\/doi.org\/10.1016\/S0968-0004(96)10048-7<\/a><\/span><\/li>\n
  3. Thomas, M. R. & O\u2019Shea, E. K. An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels. Proc. Natl. Acad. Sci.<\/em> 102,<\/strong> 9565\u20139570 (2005). https:\/\/doi.org\/10.1073\/pnas.0501122102<\/a><\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    PHO5pr Type: Metabolite-inducible promoter Other names: \/ Description: PHO5pr is a member of the PHO regulon, which has been researched intensively as a model for studying the relationship between chromatin structure and gene expression dynamics1. PHO5pr is upregulated in response to a lack of inorganic phosphate2, which is required for energy and nucleotide metabolism. The […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","footnotes":""},"class_list":["post-421","page","type-page","status-publish","hentry","entry"],"featured_image_src":null,"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t