{"id":402,"date":"2022-08-02T11:56:14","date_gmt":"2022-08-02T11:56:14","guid":{"rendered":"https:\/\/promoter-benchmark.epfl.ch\/?page_id=402"},"modified":"2023-06-27T19:54:08","modified_gmt":"2023-06-27T19:54:08","slug":"lip-stronglov-system","status":"publish","type":"page","link":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/lip-stronglov-system\/","title":{"rendered":"LIP-strongLOV"},"content":{"rendered":"

LIP-strongLOV<\/span><\/strong><\/span><\/h1>\n
\n\n\n\n\n\n\n\n\n\n
Type:<\/span><\/td>\nSynthetic promoter; Optogenetic promoter<\/span><\/td>\n<\/tr>\n
Other names:<\/span><\/td>\n\/<\/span><\/td>\n<\/tr>\n
Description:<\/span><\/td>\nstrongLOV is the more light-sensitive Glu84Asp mutant of the El222 transcription factor.1<\/sup>.<\/span><\/td>\n<\/tr>\n
Organism of origin:<\/span><\/td>\nThe light-responsive prokaryotic transcription factor EL222 and its binding sites come from Erythrobacter litoralis<\/em>. The transactivation domain which adapts it for eukaryotes comes from the herpes virus (Herpes simplex<\/em>) protein VP16.<\/span><\/td>\n<\/tr>\n
Available on Addgene:<\/span><\/td>\n\/<\/span><\/td>\n<\/tr>\n
Inducing condition:<\/span><\/td>\nBlue light (\u2248 465 nm)<\/span><\/td>\n<\/tr>\n
Non-inducing condition:<\/span><\/td>\nDarkness<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n\t\n\n\t\n\t\n\t
Promoter name<\/th>Leakiness [% maxGAL1]<\/th>tau-on [min]<\/th>tau-off* [min]<\/th>Maximal induction level [maxGAL1]<\/th>Stationary induction level [maxGAL1]<\/th>Initial induction speed [0.001 maxGAL1\/min]<\/th>Degradation rate*[0.01\/min]<\/th>\n<\/tr>\n<\/thead>\n
LIP-EL222 80%<\/td>0.16<\/td>2.2<\/td>12.6<\/td>0.73<\/td>0.60<\/td>3.1<\/td>1.1<\/td>\n<\/tr>\n
LIP-strongLOV 20%<\/td>0.80<\/td>1.4<\/td>46.2<\/td>0.58<\/td>0.56<\/td>2.6<\/td>1.6<\/td>\n<\/tr>\n
LIP-EL222 20%<\/td>0.16<\/td>1.3<\/td>< 1<\/td>0.20<\/td>0.16<\/td>1.3<\/td>1.0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/span><\/p>\n

*t-off and degradation rate were determined using ymScarletI as a reporter, instead of yEVenus as for the other systems. This was done because light for yEVenus excitation was strong enough to induce strongLOV.<\/p>\n

Download promoter sequence<\/a><\/strong><\/span><\/p>\n

References:<\/span><\/p>\n

    \n
  1. Gligorovski, V., Sadeghi, A., Rahi, S. J. \u201cMultidimensional single-cell benchmarking of inducible promoters for precise dynamic control in budding yeast\u201d, bioRxiv preprint 2020. https:\/\/doi.org\/10.1101\/2020.08.16.253310<\/a><\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    LIP-strongLOV Type: Synthetic promoter; Optogenetic promoter Other names: \/ Description: strongLOV is the more light-sensitive Glu84Asp mutant of the El222 transcription factor.1. Organism of origin: The light-responsive prokaryotic transcription factor EL222 and its binding sites come from Erythrobacter litoralis. The transactivation domain which adapts it for eukaryotes comes from the herpes virus (Herpes simplex) protein […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","footnotes":""},"class_list":["post-402","page","type-page","status-publish","hentry","entry"],"featured_image_src":null,"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages\/402","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/comments?post=402"}],"version-history":[{"count":0,"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/pages\/402\/revisions"}],"wp:attachment":[{"href":"https:\/\/promoter-benchmark.epfl.ch\/index.php\/wp-json\/wp\/v2\/media?parent=402"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}